Complete List of Target Analytes Measured on the GI-MAP

Bacterial pathogens:

Campylobacter

C. diff Toxin A & B**

E. coli o157**

Enterotoxigenic E. coli LT & ST (ETEC)**

Shiga-like Toxin producing E. coli stx1 & stx2 (STEC)**

Salmonella

Shigella

Vibrio cholera

Yersinia enterocolitica

Viral pathogens:

Adenovirus 40 & 41**

Norovirus GI & GII**

Rotavirus A**

Parasitic pathogens

Cryptosporidium**

Entamoeba histolytica**

Giardia

Additional targets Bacteria:

Helicobacter pylori and virulence factors, cagA and vacA**

Enterococcus

Lactobacillus**

Bifidobacter**

Bacteroides spp.

Bacteroides fragilis grp

E. coli (total) 

Citrobacter spp.

Citrobacter freundii

Proteus spp.

Proteus mirabilus

Proteus vulgaris

Pseudomonas spp.**

Pseudomonas aeruginosa

Morganella spp.

Staphylococcus spp. (aureus)

Streptococcus spp.

Klebsiella spp.**

Klebsiella pneumoneiae

Parasites:

Blastocystis hominis**

Dientamoeba fragilis

Endolimax nana

Entamoeba coli**

Entamoeba hartmanni**

Chilomastix mesnelli

Cyclospora cayetanenensis

Pentatrichomonas hominis LDT

fungi/yeast:

Microsporidia spp. including Enterocytozoon bieneusi and Encephalitozoon intestinalis ** Candida albicans

Candida spp.

Geotrichum spp.

Trichosporon spp.

Other tests:

Secretory IgA (sIgA)

Anti-gliadin sIgA

Pancreatic elastase 1

Lactoferrin

Occult blood


*These statements have not been evaluated by the Food and Drug Administration. These products are not intended to diagnose, treat, cure or prevent any disease.  Organisms with ** are listed with citations in this paper.

GI-MAP TESTING

DNA Stool Analysis

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The GI-MAP can be used in the detection and identification of gastrointestinal microbial nucleic acids and has been clinically validated for the detection of gastrointestinal pathogens that cause infectious colitis or gastroenteritis.1 This technology has been used to identify and control pathogen outbreaks because of its rapid turn-around-time. 1 It measures a substantial list of opportunistic pathogens as well as a list of FDA-cleared pathogens, including novel targets such as viruses, Microsporidia, and pathogenic virulence factors. Chronic gastrointestinal symptoms, intestinal permeability, hormonal imbalance, and food sensitivities may trace their origins to imbalanced gut microbes as a root cause. Further, chronic inflammatory arthritis could have a microbial component that may warrant investigation by stool studies. This stool test offers integrative and functional medicine practitioners superior sensitivity and specificity to help resolve persistent and complex illnesses. Since the immune system, the intestinal barrier, and microbial diversity are intimately interwoven, thorough understanding of our gut microbiome holds promise for new approaches to treat and prevent disease.122

• IVD Pathogen Assay

• True quantitation

• Rapid turn-around time (3-5 days)

• True molecular analysis

 The GIMAP is the first comprehensive stool analysis to include an FDA approved

DNA/PCR assay for GI pathogens performed on stool.

GI-MAP tests for GI pathogens, opportunistic organisms,

normal flora, and markers of GI health including:

• Bacteria

• Viruses

• Parasites

• Fungi

• Inflammation

• Immune function

• Pancreatic function

• Occult blood 

DNA/PCR techniques also allow for the measurement of antibiotic resistance genes and virulence factors that contribute to pathogenicity. Both are used to help determine clinical significance and the most effective treatments. Other available stool tests may appear to be DNA-based tests, but they are in fact only using Matrix Assisted Laser Desorption/ Ionization Time-of-Flight (MALDI-TOF) to identify stool microbes. Their methodology relies on cultures from stool and are not molecular/PCR based assays. With a culture based test it is not possible to measure strict anaerobes, viruses or virulence factors. They also lack the ability to deliver true quantitation of any of the reported organisms. There have been many advances in DNA technology over the last 10 years, including an FDA approved, PCR based technology and assay that is used by major hospital labs. Considering these advances, why would you still rely on culture and microscopy for stool analysis and assessment of the GI environment?


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